高分悬赏，中文翻译成英语，机器翻译的不要

翻译如下：Preparation of coenzyme Q10 precursor liposome 1 emulsion evaporation combined with high-pressure homogenization method

Precursor liposome (proliposome), also known as the reconstruction of liposome, the precursor form of liposome, usually dry and have good flow properties of the powder, the precursor liposome generally do not have the integrity of the bilayer lipid membrane. But the bilayer lipid membrane fragments or lipid membrane attached to a support agent crystallization, or with the support agent are evenly mixed, application and water hydration can form intact liposomes. It has a series of characteristics of liposome preparations, and can solve the problem of easy hydrolysis, aggregation, hierarchical, the drug leakage and high-temperature sterilization is not stable, laid the basis for the design for the industrial production of liposomes, so at home and abroad has aroused extensive research on [120-121].

Preparation method is simple, storage stability than liposomes corresponding suspension to improve, will open up new practical significance of enlarging the production and application of liposomes. But the main reason proliposome commercialization process is still relatively slow is

(1) Proliposomes quality is difficult to control, such as by dilution or rehydration preparation of liposome size distribution is not uniform enough (2)

The drug entrapment efficiency is not up to the requirements. (3) targeting remains to be improved, this is mainly because in the organism, the size of liposome size determines its in vivo and cell function and the location of the in vivo absorption and distribution. Such as the condition of intravenous injection, liposomes small can reach the liver cells soon; liposome size to maintain a considerable period of time in the blood circulation; larger liposomes can not reach the part receiving the medicine [122-123].

For all of these reasons, we have decided to adopt emulsion evaporation to prepare coenzyme Q10 precursor liposome with high pressure homogenization method. This method is based on modified emulsion evaporation method: first the formation of O/W emulsion, the organic solvent volatile after decompression, internal medicine crystallization in single phospholipid membrane, because the structure is not stable, and the film side invagination, finally ends into a double membrane structure. The formation of the liposomes modified high pressure homogenization particle size, adding additional agent after freeze drying to obtain proliposome powder dry. This method can not only obtain the precursor liposome nanometer level, in order to improve the targeting and product quality stability, but also can prevent the lipid oxidation, simple operation, easy industrialization production.

1.1 experimental materials and equipment of RE-52AA type rotary evaporator, Shanghai Qingpu Huxi instrument factory; KQ-250DB NC Ultrasonic cleaner, Gongyi Yuhua Instrument Co., Ltd.; Scientz-10N type vacuum freezing dryer, Ningbo scientz biotechnology Polytron Technologies Inc; NS1001L

High pressure homogenizer, Italy GEA Niro Soavi; Biofuge 22R type low speed centrifuge, the German company Heraeus

等了很久都不见高手现身，只好由我献丑了！！

The preparation of coenzyme Q10 Pronanoliposomes by applying the combined methods of
emulsion solvent evaporation technique and high pressure homogenization.

Proliposome, also called Reconstituted Liposome, it is the precursor form of liposome, generally it is in dry powder form with excellent fluidity. This kind of proliposome usually does not have a complete bilayer lipoid membrane, rather, it is the fragments of double molecular lipoid membrane or lipoid membrane materials attached to propping agent crystals, or homogenizes with propping agent; before application, it hydrates with water to form
complete liposome. Proliposome possesses a series of action characteristics of liposome preparation, and solves the problems of easy hydrolysis, aggregation, stratification, drug leakage and unstable heat sterilization; these have laid the design foundation for the commercial production of liposome, and given rise to extensive researches here and abroad. [120-121]

The preparation method of proliposome is relatively simple; the storage stability is much better than liposome suspension, this has a practical pioneering significance on the expanded
production and application of liposome. However, the process of commercialization of proliposome is still very slow up to now, the reasons are: 1) The quality of proliposome is not easy to control; 2) The entrapment efficiency is often not up to requirements; 3) The targeting leaves much to be desired; this is mainly because in the body of living creatures, the particle size of liposome decides its action location with the cells in the body, as well as the absorption and distribution. For example, under the condition of intravenous injection, the smaller liposome can quickly reach the liver cells; the medium size liposome can remain in blood circulation for quite some time; but the larger liposome cannot reach the administration location [122-123].

Based on the above reasons, this research decides to prepare coenzyme Q10 Pronanoliposomes by adopting the combined methods of emulsion solvent evaporation technique and high pressure homogenization. This modified technique is based on emulsified evaporation method: Initially create o/w typed emulsion, after the complete decompression

of the organic solvent, due to the unstable structure, the drug crystallization within the phospholipid membrane causes one side of the membrane to invaginate, and finally the two ends fuse together and become a bi-layer membrane structure. After the created liposome has been subjected to high pressure homogenization and changed into grain sizes, and the proliposome in dry powder form is obtained after freeze-dried by adding additional agent. This process not only can obtain proliposome in nano-scale which can enhance the targeting and the stability of product quality, but can also prevent atmospheric oxidation of the phospholipid, and the operation is simple and convenient, suitable for industrial production.

1.1 Experiment materials and equipment: Rotary evaporator model RE-52AA (Shanghai Qingpu Huxi Instrument Factory), CNC Ultrasonic Cleaner model KQ-250DB (Gongyi Yuhua Instrument Co., Ltd.), vacuum freeze dryer model Scientz-10N (Ningbo scientz biotechnology Polytron Technologies Inc), High pressure homogenizer NS 1001L (GEA Niro Soavi Company, Italy), Hypothermic high speed centrifugal machine model Biofuge 22R (Heraeus Company, Germany).

【英语牛人团】

1. Use the emulsification-evaporation process in combination with the high pressure homogenization (HPH) method to prepare the Q10 nano proliposome

The proliposome is also known as the reconstruction liposome and a pro form of liposome in a state of dry powder with good fluidity. Generally this type of proliposome doesn’t have complete lipid bi-layers but contains the fragments of it, or it is a kind of liposome with the membrane material adhering to the proppant crystallization, or which can be formed into the complete liposome when mixed uniformly with the proppant through hydration before being applied. It not only has the functions of other liposome preparations, but can also solve the instability problem caused by hydrolysis, aggregation, layering, drug leakage and high temperature sterilization. It has laid down the groundwork for industrial use of the liposome and led to extensive research both in China and the world.

The relatively simple method for preparing the proliposome as well as the higher storage stability than the liposome suspension is practically meaningful for the mass production and wider application of the liposome. As of now, however, the commercialization of the proliposome is still rather slow mainly for the following reasons: 1) the proliposome quality is hard to control. For example, the liposome may not have uniform particle size distribution (PSD) when made only through the dilution or rehydration process; 2) the drugs often fail to meet the required encapsulation efficiency, and 3) the drug targeting needs to be improved. This is because in organisms, where the liposome can take effect on cells and how fast it can distribute in and absorbed by the body are dependent on the particle size of the liposome. For example with intravenous injection, the small liposome particles can quickly reach the liver cells; the medium sized particles can remain in the blood circulation for a considerable length of time, but the large liposome can hardly get to the targeted area.

With the above reasons summarized, the researcher has decided to use the emulsification-evaporation process together with the HPH method to prepare the coenzyme Q10 nano proliposome. This improved method is based on the emulsification-evaporation process: first we prepare the O/W type emulsion, and then wait for the drug to crystallize inside the lecithoid monolayer when the organic solvent is completely evaporated. Because this is an instable structure which causes one side of the layer to invaginate, finally both ends will fuse to form a bi-layer structure. The dry proliposome powder can be obtained from the liposome thus produced with its particles resized through the HPH process and frozen dried by additives. The process can not only produce the nano-level proliposome to improve targeting and product quality, but also has advantages such as protecting phospholipids against oxidation, simple to handle and easy for industrial production.

1.1 Material and equipment used in experiments: RE-52AA Rotary Evaporator (Shanghai Qingpu Huxi Instrument Plant); KQ-250DB NC Ultrasonic Cleaner (Gonyi Yuhua Instrument Co., Ltd.); Scientz-10N Vacuum Freeze Drier (Ningbo Xinzhi Biotech Co., Ltd.); NS1001L High Pressure Homogenizer(GEA Niro Soavi, Italy); Biofuge 22R Low-temperature High Speed Centrifuge (Heraeus, Germany)

不行，跳过了。准确翻译可能得要4-10个小时，除非是双语专业人士。

分是挺高啊，不过翻译挺费劲，建议使用机器翻译的基础上自己在进行修改，这样会比较快一些！