哪位大虾能帮小弟翻译下这篇摘要,急,毕业论文要用的,谢谢!

2024年11月17日 14:44
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SOD marine yeast Purification of a preliminary research

Abstract : marine yeast for materials and the use of toluene, ethanol-chloroform, isopropanol and ultrasonic its broken; SOD isolated from crude enzyme solution, using pyrogallol autoxidation, Determination of different methods under broken SOD enzyme activity, select the highest activity of toluene; Toluene used to cell was broken by the enzyme regulating pH protein removal, acetone secondary precipitation, SOD be purified, to live more than 1,219 U / mg, yield of 5.6%; at 25 ° C to 95 ° C between the SOD heat for experiments, The results showed less than 55 ° C, SOD activity was basically stable, at 65 ° C can maintain 79.64% of the activity; SOD purified by CHCl3-ethanol, hydrogen peroxide inhibition test, the enzyme types of Cu, Zn - SOD; 200 ~ 400 nm in the UV-determination under the maximum absorption wavelength, 260 nm see the largest OD value of 0.931, showing peaks and proof-type Cu, Zn - SOD.

Keywords : superoxide dismutase; Ocean yeast; Broken; Purification; SOD type